Coding

Part:BBa_K3168009:Design

Designed by: Simone Wouters   Group: iGEM19_TU_Eindhoven   (2019-09-30)


mNeonGreen-NanoLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 739
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains a His-tag in the front and a Strep-tag in the end for protein purification


Source

NanoLuc is a deep-sea shrimp-derived luciferase while mNeonGreen is derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. This part is a fusion protein of these two proteins.

References