Coding
Part:BBa_K3168009:Design
Designed by: Simone Wouters Group: iGEM19_TU_Eindhoven (2019-09-30)
mNeonGreen-NanoLuc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 245
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 739
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence contains a His-tag in the front and a Strep-tag in the end for protein purification
Source
NanoLuc is a deep-sea shrimp-derived luciferase while mNeonGreen is derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. This part is a fusion protein of these two proteins.